Characterization of the Lectin from the Bulbs of Eranthis hyemalis
نویسنده
چکیده
From the $Department of Biotechnology Research, Pioneer Hi-Bred International, Inc., Johnston, Iowa 50131, the §Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106, the llDepartment of Biological Chemistry, University of Health Sciences/Chicago Medical School, Chicago, Illinois 60064, 11 Hematology Research, Department of Biochemistry and Molecular Biology, Mayo Clinic and Foundation, Rochester, Minnesota 55905, and **Laboratorium voor Plantenbiochemie, Katholieke Universiteit Leuuen, Willem De C r o y h n 42, Heuerlee, Belgium
منابع مشابه
Isolation and partial characterization of an N-acetylgalactosamine-specific lectin from winter-aconite (Eranthis hyemalis) root tubers.
A lectin was isolated from root tubers of winter aconite (Eranthis hyemalis) by affinity chromatography on fetuin-agarose, and it was partially characterized with respect to its biochemical, physicochemical and carbohydrate-binding properties. The Eranthis hyemalis lectin is a dimeric protein (Mr 62000) composed of two different subunits of Mr 30000 and 32000, held together by disulphide bonds....
متن کاملWinter Aconite (Eranthis hyemalis) Lectin as a cytotoxic effector in the lifecycle of Caenorhabditis elegans
The lectin found in the tubers of the Winter Aconite (Eranthis hyemalis) plant is an N-acetyl-D-galactosamine specific Type II Ribosome Inactivating Protein (RIP); Type II RIPs have shown anti-cancer properties, and hence have potential as therapeutic agents. Here we present a modified protocol for the extraction and purification of the E. hyemalis lectin (EHL) using affinity chromatography. De...
متن کاملGenes from the APETALA3 and PISTILLATA lineages are expressed in developing vascular bundles of the tuberous rhizome, flowering stem and flower Primordia of Eranthis hyemalis.
In Arabidopsis thaliana expression of the B-class MADS-box genes APETALA3 (AP3) and PISTILLATA (PI) is confined to petals and stamens but in other plant species these genes are also transcribed in non-flower tissues; in Solanum tuberosum they are transcribed specifically in vascular bundles leading to petals and stamens. Transcription analysis of B-class genes in Eranthis hyemalis using reverse...
متن کاملA New Galactose-Specific Lectin from Clerodendrum infortunatum
Background: The ethno-medical significance of Clerodendrum genus raises the interest towards the characterization of its seed lectin by inexpensive and most effective technique.Objective: The focus of this study is the purification, characterization, and evaluation of the antioxidant and antiproliferative potential of a galactose-specific lectin from Cler...
متن کاملLectin Histochemistry Assessment of Seminiferous Epithelium Glycoconjugates in Mouse
Purpose: Plasma membrane glycoconjugates are important determinantsof differentiation and iteraction during spermtogenesis. Which entails miotic and meiosis proliferation, alternation in cell shape and so on. Hihg rates of them synthetized and present in acrosomes. With attention to such importancy, the present study has was designed to analyze glycoconjugates with special terminal sugar residu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2001